po1f strain  (ATCC)

Journal: Frontiers in Fungal Biology

Article Title: First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica

doi: 10.3389/ffunb.2024.1327777

Figure Lengend Snippet: Overview of heterologous production of polyketide-derived compounds in Y. lipolytica .

Article Snippet: Yarrowia lipolytica strain PO1f (MatA, ura3-302, leu2-270, xpr2-322, axp-2), ATCC MYA-2613 ( ) was used as parental strain for inserting the 6-MSAS encoding gene, bostrycoidin gene cluster, together with the co-activating PPTase.

Techniques:

Journal: Frontiers in Fungal Biology

Article Title: First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica

doi: 10.3389/ffunb.2024.1327777

Figure Lengend Snippet: Visual confirmation of bostrycoidin biosynthetic pathway integration. (A) Wild-type strain of Y. lipolytica. (B—G) Strains expressing the bostrycoidin pathway. A difference between the non-curated (B, D, F) and donor-plasmid-curated strains (C, E, G) could be observed, confirming that Y. lipolytica requires curation with 5-FOA to eliminate the URA3-containing donor plasmid.

Article Snippet: Yarrowia lipolytica strain PO1f (MatA, ura3-302, leu2-270, xpr2-322, axp-2), ATCC MYA-2613 ( ) was used as parental strain for inserting the 6-MSAS encoding gene, bostrycoidin gene cluster, together with the co-activating PPTase.

Techniques: Expressing, Plasmid Preparation

Journal: Frontiers in Fungal Biology

Article Title: First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica

doi: 10.3389/ffunb.2024.1327777

Figure Lengend Snippet: (A) Chromatograms of a 6-MSA and bostrycoidin standard of 25 mg/L and extracts from Y. lipolytica transformants, both containing 31 mg/L 6-MSA and bostrycoidin, respectively. (B) Standard curves of 6-MSA and bostrycoidin in a twofold dilution series (0.02 - 100 mg/L).

Article Snippet: Yarrowia lipolytica strain PO1f (MatA, ura3-302, leu2-270, xpr2-322, axp-2), ATCC MYA-2613 ( ) was used as parental strain for inserting the 6-MSAS encoding gene, bostrycoidin gene cluster, together with the co-activating PPTase.

Techniques:

Journal: Frontiers in Fungal Biology

Article Title: First-class – biosynthesis of 6-MSA and bostrycoidin type I polyketides in Yarrowia lipolytica

doi: 10.3389/ffunb.2024.1327777

Figure Lengend Snippet: Strain engineering for optimization of 6-MSA and bostrycoidin biosynthesis in Yarrowia lipolytica. Overview of the main processes in the lipid metabolism of Y. lipolytica – the synthesis of lipid precursors, lipogenesis, and lipid degradation are color-coded green, purple, and blue, respectively. Recycling of lipid/polyketide building blocks, acetyl- and malonyl-CoA, is attempted by up-regulating ß-oxidation through overexpression of TGL4 and AOX2 (highlighted in bold). Abbreviations and enzyme names: AcCoA, acetyl-CoA; MalCoA, malonyl-CoA; PDH, pyruvate dehydrogenase complex; TCA, tricarboxylic acid; ACL1 and ACL2, ATP-citrate lyases; ACC1, acetyl-CoA carboxylase; FAS, fatty acid synthase complex; TGL3 and TGL4, lipases; AOX1-6, acyl-CoA oxidases (encoded by POX1-6 genes); MFE2, multi-functional enzyme; POT1, thiolase.

Article Snippet: Yarrowia lipolytica strain PO1f (MatA, ura3-302, leu2-270, xpr2-322, axp-2), ATCC MYA-2613 ( ) was used as parental strain for inserting the 6-MSAS encoding gene, bostrycoidin gene cluster, together with the co-activating PPTase.

Techniques: Over Expression, Functional Assay

Journal: Bioresources and Bioprocessing

Article Title: De novo synthesis of nervonic acid and optimization of metabolic regulation by Yarrowia lipolytica

doi: 10.1186/s40643-023-00689-6

Figure Lengend Snippet: Strains used in this study

Article Snippet: Yarrowia lipolytica strain ATCC MYA2613 (Po1f) was used as the initial strain of the engineered strains.

Techniques: CRISPR